Lipooligosaccharide Ligands from Respiratory Bacterial Pathogens Enhance Cellular Uptake of Nanoparticles

Several bacterial pathogens contain membrane ligands that facilitate their binding and internalization into human tissues. In this study, lipooligosaccharides (LOS) from the respiratory pathogen non-typeable Haemophilus influenzae (NTHi) were isolated surface evaluated as a nanoparticle coating material to uptake epithelium. NTHi clinical isolates screened select strain with high potential due elevated phosphorylcholine content. The association of particles bronchial epithelial cells was investigated function particle chemistry incubation time, mechanism via chemical inhibitor receptor activation studies. A more than two-fold enhancement in achieved by LOS compared uncoated or gelatin-coated particles, which further increased activating platelet factor (PAFR). These findings demonstrate bacterial-derived can enhance targeting nanoparticles lung cells.